phenol red cell culture

PubChem Substance ID 24898424. Phenol red Why is phenol red added to cell culture medium? Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. At low pH levels, it turns the media yellow, while at higher pH levels it turns the media purple. Image credit: Stephanie Gamez (University of California San Diego, La Jolla, CA). Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates. By switching countries your current shopping cart will be cleared. A solution of phenol red is used as a pH indicator, often in cell culture. Enter multiple addresses on separate lines or separate them with commas. Contact Us Privacy Policy Ancestors of domestic cats may have existed in a commensal but not yet domesticated relationship with Neolithic farmers. Beilstein/REAXYS Number 326470 . Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. Quality Accreditations. Unfortunately, phenol red can mimic the action of some steroid hormones, particularly estrogen. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Are you sure? A pH indicator changing colour from yellow below pH 6.8 to bright pink above pH 8.2, it is commonly used as an indicator in cell cultures and in … Phenol red-free formulations are recommended for assays that require color detection. Happy Thanksgiving! Phenol red is an acid-base indicator. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. Image credit: Michał Wojenka and Magdalena Krajcarz. Phenol red is used as a pH indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol Red is used as a pH indicator in cell culture applications. Offer ends Nov. 30th, 2020. The estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture. To design an estrogen and phenol red free medium for cell culture and check its effectiveness and safety on osteoblast growth it is necessary to maintain the estrogen receptors free for tests. Enter your user name and click Submit. For the best experience on our site, be sure to turn on Javascript in your browser. For most tissue culture work (pH 7.4), the media is bright red. We have found that phenol red, which bears a structural resemblance to some nonsteroidal estrogens and which is used ubiquitously as a pH indicator in tissue culture media, has significant estrogenic activity at the concentrations (15-45 microM) at which it is found in tissue culture media. MDL number MFCD00003552. A small fossil reptile related to dinosaurs and pterosaurs suggests a miniaturized origin for some of the largest animals to live on Earth. It is typically used in cell culture media at 11 mg/L. Please select a country, so we can supply you with the most accurate content available for your country or region. Additionally, the sodium-potassium ion homeostasis is upset when phenol red is included in some serum-free formulations; this affect is neutralized by the inclusion of serum or bovine pituitary hormone in the medium (S Karmiol, 2000, Development of serum free media, in Animal Cell Culture, 3rd edition, J.R.W. NACRES NA.47 David Crowder and Gengping Zhu explain how to predict the spread of the Asian giant hornet. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. © 2020 ATCC. Phenol red is frequently omitted from studies using flow cytometry, as its color interferes with detection. An email will be sent to you with instructions. Phenol red is 3H-2,1-Benzoxathiole 1,1-dioxide in which both of the hydrogens at position 3 have been substituted by 4-hydroxyphenyl groups.   |   All rights reserved. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red-free formulations are manufactured using DMEM that does not contain phenol red, so as a result the product is colorless. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above.   |     |   DNA Extraction from Difficult to Grind Plants, Infectious Disease Testing - Hepatitis E Virus, Cell Culture Reagents for Cancer Research, Tissue Homogenization and Cell Culture Lysis, Skip to the beginning of the images gallery, Liquid | Phenol Red Sodium Salt: 5000.00000 mg/L, Visual transition interval is from pH 6.8 (yellow) to pH 8.2 (red). Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates. Although much attention has been paid to the removal of hormones from sera and to the development of serum-free media for studies on hormone-responsive cells in culture, little consideration has been given to the possibility that the media components themselves may have hormonal activity. Studies with estrogen-sensitive cells, such as mammary tissue, use media without phenol red. Online ISSN 1091-6490. All Rights Reserved. JavaScript seems to be disabled in your browser. It stimulates the proliferation of estrogen receptor-positive MCF-7 breast cancer cells in a dose-dependent manner but has no effect on the growth of estrogen receptor-negative MDA-MB-231 breast cancer cells. For the best experience on our site, be sure to turn on Javascript in your browser.

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